FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76972
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11991.5577092968871797No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA11051.4355869666891856No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10461.3589357168840617No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA9691.2588993400197475No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8221.067920802369693No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA8071.0484331964870344No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8001.0393389804084603No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7771.0094579847217169No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7470.9704827729563997No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA7100.9224133451125084No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6770.8795406121706595No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6730.8743439172686172No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6570.8535571376604479No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6540.8496596164839162No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6360.8262744894247258No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA5770.7496232396196019No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5490.7132463753053059No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5210.6768695109910098No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA5200.6755703372654991No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5180.672971989814478No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA4900.6365951255001819No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4900.6365951255001819No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4790.6223042145195655No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4720.6132099984409914No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4690.6093124772644598No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4680.6080133035389492No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4670.6067141298134386No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA4630.6015174349113963No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4570.5937223925583329No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4550.5911240451073118No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4310.559943875695058No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA4230.5495504858909733No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4190.5443537909889311No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3990.5183703164787196No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3900.5066777529491244No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3660.47549758353687055No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3580.46510419373278594No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3570.46380502000727536No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3410.44301824039910614No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3370.43782154549706387No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3330.43262485059502154No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA3270.4248298082419581No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3250.422231460790937No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3220.4183339396144053No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3220.4183339396144053No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3220.4183339396144053No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3000.3897521176531726No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2990.388452943927662No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2970.38585459647664083No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2940.38195707530010914No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA2890.3754612066725563No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2850.37026451177051395No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2710.3520760796133659No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA2690.3494777321623448No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2680.34817855843683415No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2660.34558021098581304No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2630.3416826898092813No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2590.336485994907239No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2560.33258847373070727No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2560.33258847373070727No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2520.327391778828665No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2410.31310086784804864No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2390.3105025203970275No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2370.30790417294600636No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2330.30270747804396403No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2200.28581821961232656No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2160.28062152471028423No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2120.27542482980824196No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2110.2741256560827314No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2110.2741256560827314No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2080.2702281349061997No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2060.2676297874551785No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA2050.26633061372966793No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2000.2598347451021151No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1990.2585355713766045No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1980.2572363976510939No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1980.2572363976510939No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1950.2533388764745622No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1860.241646312944967No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1850.2403471392194564No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1840.23904796549394586No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1790.23255209686639297No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1760.22865457568986125No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1750.22735540196435067No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1740.2260562282388401No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1690.21956035961128723No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1680.21826118588577662No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1650.2143636647092449No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1580.20526944863067087No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1580.20526944863067087No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1560.20267110117964973No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1550.20137192745413915No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1530.198773580003118No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1520.19747440627760743No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1500.1948760588265863No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1480.19227771137556515No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1460.189679363924544No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1440.18708101647352285No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1390.18058514784596996No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1370.1779868003949488No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1320.17149093176739594No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1310.17019175804188535No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1300.16889258431637477No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1290.16759341059086422No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.16629423686535363No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1280.16629423686535363No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1280.16629423686535363No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1260.1636958894143325No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1230.15979836823780075No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1230.15979836823780075No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1230.15979836823780075No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1220.1584991945122902No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1180.15330249961024786No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1170.1520033258847373No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1160.15070415215922675No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1140.14810580470820559No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1130.146806630982695No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1100.14290910980616328No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1090.1416099360806527No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1080.14031076235514212No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1080.14031076235514212No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1070.13901158862963156No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1060.13771241490412098No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.1364132411786104No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1030.13381489372758926No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA1030.13381489372758926No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA1020.13251572000207867No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1010.1312165462765681No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1000.12991737255105754No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA990.12861819882554695No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA950.12342150392350465No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA950.12342150392350465No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA950.12342150392350465No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCA930.1208231564724835No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA920.11952398274697293No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG910.11822480902146235No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT910.11822480902146235No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA900.11692563529595178No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC880.11432728784493063No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA870.11302811411942006No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA860.11172894039390947No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG860.11172894039390947No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA850.1104297666683989No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA830.10783141921737775No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA830.10783141921737775No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA820.10653224549186718No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA820.10653224549186718No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG820.10653224549186718No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA810.10523307176635659No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA810.10523307176635659No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA810.10523307176635659No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA800.10393389804084603No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.10393389804084603No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA800.10393389804084603No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC800.10393389804084603No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG800.10393389804084603No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGACATAATCTCATT790.10263472431533543No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA780.10133555058982487No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC780.10133555058982487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2000.0294.999368
CGGTTTT108.44977E-4294.999339
ACTTCGC108.44977E-4294.99933295
GGACTAC70350.0294.142151
CTACTAG12550.0289.122864
CAAGGGT12400.0289.05187
CTACAAG12400.0289.05184
TACAAGG12400.0289.05185
CTCGGGT9400.0288.722787
ACTACCG4700.0288.722783
ACTCGGG9400.0288.722786
CTACCGG4700.0288.722784
GACTACA26450.0288.30752
ACAAGGG12450.0287.890936
TACTCGG9450.0287.195135
TCGGGTT7300.0286.917188
CGGGTAT5450.0286.88019
ACTACTA12650.0286.83733
TACTGGG5200.0286.489755
CTACTCG9500.0285.68364
Produced by FastQC (version 0.11.5)