FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122003
Sequences flagged as poor quality0
Sequence length301
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA1550.0295.000032
TCGCCCT450.0295.01
GTCGCCT450.0295.01
GCGCCTA1400.0295.02
GCGCCCT350.0295.01
GTAGCCT350.0295.01
GTGTCGG206.162372E-8295.0295
GGGCCTA1250.0295.02
CTCGCCT450.0295.01
GGCGCCT255.293259E-10295.01
ATCGCCT255.293259E-10295.01
GTCTACG157.224544E-6294.999973
AGCCTAC6100.0290.163943
GCCTACG22950.0289.215674
TACGGGA111300.0288.771337
ACGGGAG111650.0287.866128
ACCCTAC7900.0285.664553
CGCCTAC6200.0285.48393
CTACGGG114250.0284.671786
CCTACGG117850.0284.236335
Produced by FastQC (version 0.11.5)