FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81190
Sequences flagged as poor quality0
Sequence length301
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1250.0295.000032
GTCGCCT303.6379788E-12295.01
GTACCCT303.6379788E-12295.01
CGAACCT450.0295.01
TTGTCCT500.0295.01
CGCGCCT303.6379788E-12295.01
CGTTCCT303.6379788E-12295.01
TACGCCT255.275069E-10295.01
GTCACCT303.6379788E-12295.01
CTCGCCT350.0295.01
CGTACCT350.0295.01
CCGACCT350.0295.01
CGCACCT450.0295.01
TTCGCCT350.0295.01
CGATCCT157.2156436E-6295.01
ATCGCCT303.6379788E-12295.01
TCGCCCT400.0294.999971
ACGGGCA108.450564E-4294.999978
CTACGAG206.149821E-8294.999976
ACGGAGC206.149821E-8294.999978
Produced by FastQC (version 0.11.5)