FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281750
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGG108.461642E-4295.0295
GTCGCCT500.0295.01
CCCCTCG255.311449E-10295.03
TGCCCCT1100.0295.01
CGAACCT1100.0295.01
GTCGATA108.461642E-4295.0295
CCGGCCT900.0295.01
TCTTACG108.461642E-4295.04
GTCTCGT206.176742E-8295.0295
CGCGCCT700.0295.01
GCGCCTA2850.0295.02
TAGCCTA4700.0295.02
GTGGATA157.234574E-6295.0295
GCACCCT900.0295.01
CACCTAG157.234574E-6295.03
CGCCTAG108.461642E-4295.03
GTAGCCT1250.0295.01
GCCCTAG108.461642E-4295.03
CCCCCTA4450.0295.02
TACCTAG157.234574E-6295.03
Produced by FastQC (version 0.11.5)