FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64776
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.13276522168704458No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.1312214400395208No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.12967765839199705No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.1265900950969495No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.1250463134494257No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.1250463134494257No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.12041496850685439No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.11887118685933061No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.11732740521180685No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.11732740521180685No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.11732740521180685No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11578362356428308No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11578362356428308No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11578362356428308No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11578362356428308No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11269606026923552No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11269606026923552No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11269606026923552No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11269606026923552No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11115227862171174No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10960849697418797No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10806471532666419No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10806471532666419No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10806471532666419No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10652093367914041No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10652093367914041No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10652093367914041No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10497715203161666No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10497715203161666No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC680.10497715203161666No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10497715203161666No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10497715203161666No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10497715203161666No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10497715203161666No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10343337038409288No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.10343337038409288No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.10343337038409288No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10343337038409288No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.10343337038409288No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.1018895887365691No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.1018895887365691No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.1018895887365691No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.1018895887365691No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.1018895887365691No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.1018895887365691No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.1018895887365691No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.1018895887365691No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10034580708904532No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10034580708904532No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10034580708904532No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC650.10034580708904532No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10034580708904532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCTA1250.0295.000032
CAGCCCT157.208915E-6295.01
ATCCCCT255.2568794E-10295.01
ACAGCCT303.6379788E-12295.01
ATCCTCG108.446622E-4295.03
GTTGATT950.0295.0295
TTGTCCT206.1401806E-8295.01
TGGTCCT255.2568794E-10295.01
ACGCCTA550.0295.02
GTGTCGG1200.0295.0295
GGGTCCT108.446622E-4295.01
CAGTCCT255.2568794E-10295.01
AGCTCCT350.0295.01
CACACCT206.1401806E-8295.01
GTGCAAC108.446622E-4295.0295
TGACCTA1100.0295.02
CTCCCTA750.0295.02
GAAACCT303.6379788E-12295.01
GTACCTA1000.0295.02
CGCCCCT108.446622E-4295.01
Produced by FastQC (version 0.11.5)