FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82311
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10326687806004058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCT350.0295.000031
GTCGCCT350.0295.000031
CATCCCT350.0295.000031
CTCTCCT350.0295.000031
CGCTCCT350.0295.000031
CTGACCT350.0295.000031
CCCACCT350.0295.000031
ATTACCT350.0295.000031
TAATCCT350.0295.000031
ATGTCCT350.0295.000031
ACCTCCT350.0295.000031
ACGACCT350.0295.000031
TACCCCT350.0295.000031
AGACCCT350.0295.000031
ATTCCCT350.0295.000031
GCCCTCG108.450779E-4295.03
CAGCCCT157.216013E-6295.01
AGCCCCT157.216013E-6295.01
ATCCTCG108.450779E-4295.03
GTTGATT108.450779E-4295.0295
Produced by FastQC (version 0.11.5)