FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86743
Sequences flagged as poor quality0
Sequence length301
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA21932.528157891703077No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA18032.0785538890746227No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA17892.062414258211037No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15931.8364594261208396No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15131.7442329640432082No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15011.7303989947315634No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA14381.6577706558454284No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13821.5932121323910864No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12131.3983837312520895No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12101.3949252389241784No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10761.2404459149441454No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10711.2346817610642933No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10021.155136437522336No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10021.155136437522336No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8610.9925872981105104No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7680.8853740359452636No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA7530.8680815743057078No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7450.8588589280979445No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7380.8507891126661518No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6680.770090958348224No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6480.7470343428288162No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6210.7159079118776155No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5920.682475819374474No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5900.6801701578225332No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5860.6755588347186516No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA5760.6640305269589477No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA5670.6536550499752141No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5610.6467380653193917No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5500.6340569267837174No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5490.632904096007747No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA5190.5983191727286351No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA5030.5798738803131088No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4970.5729568956572865No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4940.5694984033293753No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4810.5545116032417602No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4800.5533587724657898No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA4740.5464417878099673No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4680.539524803154145No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4650.5360663108262338No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4640.5349134800502634No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4590.5291493261704114No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4500.5187738491866779No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4290.4945644028912996No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4130.47611911047577327No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4110.4738134489238325No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4070.4692021258199509No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3950.45536815650830614No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3690.4253945563330759No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3660.4219360640051647No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3630.4184775716772535No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3590.41386624857337195No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3570.41156058702143106No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3550.4092549254694903No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3540.4081020946935199No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3480.4011851100376975No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA3380.3896568022779936No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3360.3873511407260528No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3320.38273981762217124No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3310.38158698684620085No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3300.38043415607023046No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3180.36660018675858574No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3140.3619888636547041No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3110.35853037132679294No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3080.35507187899888176No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3050.3516133866709706No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA3020.34815489434305935No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2970.3423907404632074No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2970.3423907404632074No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2890.33316809425544425No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2860.32970960192753307No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2830.3262511095996219No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2810.3239454480476811No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2810.3239454480476811No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2670.30780581718409555No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2660.30665298640812516No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2620.3020416633042436No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2550.2939718478724508No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA2500.2882076939925988No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2450.28244354011274686No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2390.2755265554569245No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2380.27437372468095406No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2360.2720680631290133No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2350.2709152323530429No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2330.26860957080110215No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2280.26284541692125013No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2190.2524699399375166No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA2190.2524699399375166No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2170.25016427838557576No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2170.25016427838557576No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA2130.2455529552816942No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2070.23863597062587183No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA2060.2374831398499014No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1970.22710766286616785No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1960.22595483209019748No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1950.2248020013142271No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA1950.2248020013142271No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1940.22364917053825667No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1930.2224963397622863No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1840.21212086277855274No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1830.21096803200258235No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA1760.20289821657078957No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1750.20174538579481918No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA1680.1936755703630264No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1670.192522739587056No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1670.192522739587056No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1580.18214726260332245No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1510.17407744717152968No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1510.17407744717152968No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1490.1717717856195889No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA1430.16485480096376653No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA1430.16485480096376653No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA1390.16024347785988494No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA1390.16024347785988494No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA1370.15793781630794418No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1340.15447932398003297No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1310.1510208316521218No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1300.1498680008761514No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGTCTCA1220.1406453546683882No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1070.12335289302883229No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1050.12104723147689152No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1050.12104723147689152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)