FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105128
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA19691.8729548740582909No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA16281.5485883874895365No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA12371.1766608325089416No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA12051.1462217487253634No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA11671.1100753367323644No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA11571.1005631230499962No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA10120.9626360246556579No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA9640.9169773989802907No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA9600.9131725135073434No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA8940.8503919032037135No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA8340.7933186211095046No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA7290.6934403774446389No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6770.6439768662963244No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6680.6354158739821931No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA6570.6249524389315881No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA6090.579293813256221No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA6040.574537706415037No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5830.5545620576820638No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5580.5307815234761434No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5350.5089034320066966No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA5280.5022448824290389No Hit
GGACTACAAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA5080.48322045506430256No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA5020.4775131268548817No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4800.4565862567536717No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA4700.44707404307130355No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4490.42709839433833047No Hit
GGACTACACGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4430.42139106612890953No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA4240.40331786013241No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA4200.3995129746594627No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3890.37002511224412143No Hit
GGACTACAAGGGTATCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA3840.36526900540293733No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA3840.36526900540293733No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3800.3614641199299901No Hit
GGACTACACGGGTATCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA3630.34529335666996425No Hit
GGACTACAAGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA3500.3329274788828856TruSeq Adapter, Index 27 (96% over 26bp)
GGACTACTAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3400.3234152652005175No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3390.32246404383228067No Hit
GGACTACACGGGTATCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA3370.320561601095807Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA3290.31295183014991246No Hit
GGACTACAAGGGTATCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA3200.30439083783578114Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACCCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3150.2996347309945971No Hit
GGACTACTCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3100.294878624153413No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA3030.28822007457575527No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3030.28822007457575527No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA2860.2720493113157294No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2820.26824442584278213No Hit
GGACTACAAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2800.26634198310630847No Hit
GGACTACTAGGGTATCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA2590.24636633437333536Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACACGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA2570.24446389163686172TruSeq Adapter, Index 27 (96% over 26bp)
GGACTACCGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2540.24161022753215128No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA2520.23970778479567764No Hit
GGACTACACGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2440.2320980138497831No Hit
GGACTACCCGGGTATCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA2430.23114679248154632Illumina PCR Primer Index 3 (96% over 27bp)
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2400.22829312837683585No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2360.2244882429038886No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA2340.22258580016741497No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2340.22258580016741497No Hit
GGACTACTCGGGTATCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA2330.22163457879917814No Hit
GGACTACCCGGGTATCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA2320.22068335743094133No Hit
GGACTACTGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2260.21497602922152045No Hit
GGACTACTAGGGTATCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA2240.21307358648504682No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA2240.21307358648504682No Hit
GGACTACTCGGGTATCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA2230.21212236511680996Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA2210.21021992238033632No Hit
GGACTACCGGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA2200.20926870101209955Illumina PCR Primer Index 6 (96% over 25bp)
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2190.2083174796438627No Hit
GGACTACTAGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA2150.20451259417091547Illumina PCR Primer Index 6 (96% over 25bp)
GGACTACTCGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA2080.19785404459325773Illumina PCR Primer Index 10 (96% over 25bp)
GGACTACCGGGGTATCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA1960.18643938817441594No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1960.18643938817441594No Hit
GGACTACCGGGGTATCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA1920.18263450270146867Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACTAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1800.1712198462826269No Hit
GGACTACTGGGGTATCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA1770.16836618217791643TruSeq Adapter, Index 25 (96% over 27bp)
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1770.16836618217791643No Hit
GGACTACTGGGGTATCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA1770.16836618217791643No Hit
GGACTACAAGGGTTTCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA1760.16741496080967963No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA1760.16741496080967963No Hit
GGACTACACGGGTTTCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA1760.16741496080967963No Hit
GGACTACTCGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1740.165512518073206No Hit
GGACTACCCGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA1740.165512518073206Illumina PCR Primer Index 2 (96% over 26bp)
GGACTACACGGGTATCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1700.16170763260025875Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA1660.15790274712731148No Hit
GGACTACAAGGGTATCTAATCAAGCAGAAGACGGCATACGAGATCACTGC1590.15124419754965374Illumina PCR Primer Index 5 (96% over 29bp)
GGACTACCCGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1580.15029297618141693No Hit
GGACTACAAGGGTATCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1570.14934175481318013Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1530.14553686934023286No Hit
GGACTACAAGGGTTTCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1530.14553686934023286TruSeq Adapter, Index 27 (96% over 26bp)
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA1520.14458564797199605No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1510.14363442660375925No Hit
GGACTACCCGGGTTTCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA1490.1417319838672856No Hit
GGACTACTGGGGTTTCTCGTATGCCGTCTTCTGCTTGGCTGCCTCCCGTA1490.1417319838672856Illumina PCR Primer Index 2 (96% over 26bp)
GGACTACACGGGTTTCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1490.1417319838672856TruSeq Adapter, Index 27 (96% over 26bp)
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA1470.13982954113081197No Hit
GGACTACAAGGGTATCTAATGCCGTCTTCTGCTTGTCTGCTGCCTCCCGT1460.13887831976257514No Hit
GGACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCA1460.13887831976257514No Hit
GGACTACACGGGTATCTAATGCCGTCTTCTGCTTGTCTGCTGCCTCCCGT1440.1369758770261015No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1430.1360246556578647No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1410.13412221292139107No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1370.1303173274484438No Hit
GGACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCA1370.1303173274484438No Hit
GGACTACTCGGGTTTCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA1360.129366106080207No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1360.129366106080207No Hit
GGACTACTGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1320.12556122060725972No Hit
GGACTACCAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1300.1236587778707861No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1250.118902671029602No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1250.118902671029602No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA1240.11795144966136518No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1210.11509778555665473No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1190.11319534282018111No Hit
GGACTACTCGGGTATCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1190.11319534282018111Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACTAGGGTTTCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1190.11319534282018111Illumina PCR Primer Index 6 (96% over 25bp)
GGACTACTAGGGTTTCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA1180.1122441214519443No Hit
GGACTACCGGGGTATCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1140.10843923597899705Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACAAGGGTATCTAATGCCGTCTTCTGCTTGCTCGGTGGTCGCCGT1140.10843923597899705No Hit
GGACTACCCGGGTATCTAATGCCGTCTTCTGCTTGTCTGCTGCCTCCCGT1140.10843923597899705No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1130.10748801461076023No Hit
GGACTACCGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1130.10748801461076023No Hit
GGACTACACGGGTATCTAATCAAGCAGAAGACGGCATACGAGATCACTGC1120.10653679324252341Illumina PCR Primer Index 5 (96% over 29bp)
GGACTACTAGGGTATCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1110.10558557187428659Illumina PCR Primer Index 2 (96% over 27bp)
GGACTACCCGGGTATCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1100.10463435050604977Illumina PCR Primer Index 3 (96% over 27bp)
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA1090.10368312913781295No Hit
GGACTACAGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1080.10273190776957614No Hit
GGACTACCGGGGTTTCTAATGCCGTCTTCTGCTTGCTGCTGCCTCCCGTA1080.10273190776957614No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1070.10178068640133932No Hit
GGACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCA1070.10178068640133932No Hit
GGACTACACGGGTATCTAATGCCGTCTTCTGCTTGCTCGGTGGTCGCCGT1070.10178068640133932No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1060.1008294650331025No Hit
GGACTACCGGGGTTTCTCGTATGCCGTCTTCTGCTTGCTGCCTCCCGTAG1060.1008294650331025Illumina PCR Primer Index 6 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCCT255.275069E-10294.99671
CTAAGGG700.0294.996674
TATAGGG206.158734E-8294.996675
GGACTAC93000.0294.520871
CAAGGGT17450.0293.306187
ACAAGGG17550.0292.47536
TACCCGG9700.0291.955475
CTGGGGT7900.0291.262547
ACCGGGG10000.0290.571726
TACTAGG11500.0289.86635
CTACCCG10700.0289.482734
ACTGGGG7900.0289.395486
ACCCGGG9800.0288.976326
CACGGGT15300.0288.248387
ACACGGG15250.0288.226266
TACCGGG10550.0288.006235
GACTACT30150.0287.658422
CTAGGGT11450.0287.267527
TACAGGG3700.0287.023775
TACTCGG10600.0286.64775
Produced by FastQC (version 0.11.5)