FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67378
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.13060642939831993No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.12615393748701356No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.12615393748701356No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.12466977351657811No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.12170144557570721No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.12170144557570721No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.11873311763483631No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.11576478969396539No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.11576478969396539No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11428062572352994No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.11279646175309448No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.11279646175309448No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.11279646175309448No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.11279646175309448No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.11279646175309448No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.11279646175309448No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10982813381222357No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10982813381222357No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10982813381222357No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.10834396984178814No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.10834396984178814No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.10834396984178814No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.10834396984178814No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.10834396984178814No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10685980587135267No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10685980587135267No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10685980587135267No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10537564190091721No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC700.10389147793048177No Hit
ACAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC700.10389147793048177No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC700.10389147793048177No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC700.10389147793048177No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.1024073139600463No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.1024073139600463No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.1024073139600463No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.1024073139600463No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.1024073139600463No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.1024073139600463No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.1024073139600463No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.1024073139600463No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.10092314998961084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1150.0295.02
GCCCCTA850.0295.02
CTGTCCT206.1419996E-8295.01
GTCGCCT255.2568794E-10295.01
TGCCCCT303.6379788E-12295.01
CGAACCT255.2568794E-10295.01
ACCATGT108.447376E-4295.0295
TAGACCT255.2568794E-10295.01
GCGCCTA950.0295.02
CCTACGA108.447376E-4295.05
GACCCTA1000.0295.02
ACGGGCG108.447376E-4295.07
ACCTCGG157.2101993E-6295.04
TTCCCTA900.0295.02
ACCTAGG157.2101993E-6295.04
TACGCCT206.1419996E-8295.01
TGCGCCT303.6379788E-12295.01
CTACGGC108.447376E-4295.06
CTACGAG108.447376E-4295.06
GAGCCTA1000.0295.02
Produced by FastQC (version 0.11.5)