FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62356
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1010.1619731862210533No Hit
TTCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1000.16036949130797357No Hit
TAGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT960.15395471165565464No Hit
ATATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.15074732182949516No Hit
TTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT930.14914362691641542No Hit
ATCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT920.14753993200333568No Hit
TAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT890.1427288472640965No Hit
TACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT890.1427288472640965No Hit
ACTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT880.14112515235101675No Hit
TACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT880.14112515235101675No Hit
GCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.139521457437937No Hit
TTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.139521457437937No Hit
TTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.139521457437937No Hit
CTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.13791776252485727No Hit
TTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.13631406761177753No Hit
AACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.13631406761177753No Hit
ATTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.13631406761177753No Hit
TTACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.13631406761177753No Hit
ATTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT840.13471037269869782No Hit
CAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT840.13471037269869782No Hit
TCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT840.13471037269869782No Hit
CTCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT830.13310667778561805No Hit
ACATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT830.13310667778561805No Hit
AACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT830.13310667778561805No Hit
TCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT820.13150298287253834No Hit
TCGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT820.13150298287253834No Hit
CTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.12829559304637886No Hit
ACCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.12829559304637886No Hit
TATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.12829559304637886No Hit
GGATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.12829559304637886No Hit
TCACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.12829559304637886No Hit
TTGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.12829559304637886No Hit
TACCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.12669189813329912No Hit
CACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.12669189813329912No Hit
AGCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.12669189813329912No Hit
GTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT780.12508820322021938No Hit
GGCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT780.12508820322021938No Hit
AAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT780.12508820322021938No Hit
TATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT780.12508820322021938No Hit
TCGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT780.12508820322021938No Hit
TTCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT780.12508820322021938No Hit
AAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12348450830713965No Hit
TAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12348450830713965No Hit
CAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT760.12188081339405991No Hit
TCTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT760.12188081339405991No Hit
ATCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12027711848098019No Hit
TTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12027711848098019No Hit
CTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12027711848098019No Hit
CCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12027711848098019No Hit
CATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12027711848098019No Hit
CTGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12027711848098019No Hit
CCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.12027711848098019No Hit
CTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11867342356790044No Hit
AGAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11867342356790044No Hit
GGGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11867342356790044No Hit
CCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11867342356790044No Hit
ATAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11867342356790044No Hit
CATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11867342356790044No Hit
GGTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
TGAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
GTCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
CCGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
CGAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
ATCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
TCTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
GTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
CTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
TGGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
TGCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.11706972865482071No Hit
ACGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11546603374174096No Hit
ATACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11546603374174096No Hit
GACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11546603374174096No Hit
TCCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.11386233882866123No Hit
GTACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.11386233882866123No Hit
GTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.11386233882866123No Hit
GAACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.1122586439155815No Hit
GTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.1122586439155815No Hit
GGTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.1122586439155815No Hit
AAGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT690.11065494900250177No Hit
AGGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT690.11065494900250177No Hit
TGTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10905125408942204No Hit
GATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10905125408942204No Hit
TTTCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10905125408942204No Hit
ACAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10905125408942204No Hit
TTAGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10905125408942204No Hit
TAACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10905125408942204No Hit
TTGGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10905125408942204No Hit
AATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10744755917634229No Hit
TTCGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10744755917634229No Hit
TCGGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10744755917634229No Hit
GTGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10744755917634229No Hit
GGGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10744755917634229No Hit
CGCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10744755917634229No Hit
CATCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10584386426326256No Hit
TACGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10584386426326256No Hit
CTGGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10584386426326256No Hit
TAGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10584386426326256No Hit
GATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10584386426326256No Hit
ACTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10424016935018281No Hit
ATGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10424016935018281No Hit
TTGCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10263647443710308No Hit
AGATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10263647443710308No Hit
GTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10263647443710308No Hit
TGACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10263647443710308No Hit
AGACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10263647443710308No Hit
ACGGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10263647443710308No Hit
TGATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10263647443710308No Hit
CAGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit
TATCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit
TCCGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit
TCGCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit
GCGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit
TAAGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit
TGCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit
GAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit
ATGGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit
ACGCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10103277952402336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCT350.0295.000031
CGGCGGC108.445872E-4295.09
AACCACG108.445872E-4295.03
CGACCTA800.0295.02
CGCGCCT206.1383616E-8295.01
GCGCCTA800.0295.02
ACCTCGG206.1383616E-8295.04
TCGACCT206.1383616E-8295.01
ATACCTC108.445872E-4295.02
ATCGCCT255.2568794E-10295.01
GGCTACG108.445872E-4295.03
GCCTACG11850.0290.02114
TCCTACG16500.0289.636354
CCTACGG59100.0288.76065
TACGGGA56450.0288.728977
CTACGGG57400.0287.804876
ACGGGAG56400.0287.67738
CCCTACG15050.0287.159454
ACCTACG15950.0284.827584
CCCCTAC4150.0284.337343
Produced by FastQC (version 0.11.5)