FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86364
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCT206.152004E-8295.000031
GCGCCCT108.451498E-4295.000031
CGCCTAG108.451498E-4295.000033
GGCGCCT108.451498E-4295.000031
GCTACGG108.451498E-4295.000034
TAGTCCT108.451498E-4295.000031
GTACCCT450.0295.01
GGCCCTA1050.0295.02
GAGCCTA1100.0295.02
CGAGCCT255.275069E-10295.01
CGGTCCT350.0295.01
ATCGCCT255.275069E-10295.01
TACGCCT303.6379788E-12294.999971
TAAGCCT303.6379788E-12294.999971
CGATCCT303.6379788E-12294.999971
ACCTACG23650.0291.257934
CCTACGG79850.0289.643075
TCCTACG21850.0289.599554
TACGGGA77150.0289.073247
CTACGGG78050.0288.76366
Produced by FastQC (version 0.11.5)