FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86364
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8771.0154694085498588No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8480.981890602565884No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7280.8429438191839193No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6950.804733453753879No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6600.7642073086008059No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6600.7642073086008059No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5940.6877865777407253No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5820.6738918994025288No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5700.6599972210643323No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5690.658839331202816No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5120.5928396090963828No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5060.5858922699272845No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4840.5604186929739243No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4770.5523134639433097No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4710.5453661247742114No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4670.540734565328146No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4550.5268398869899495No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4400.509471539067204No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4140.4793664026677783No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4080.47241906349867996No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4010.46431383446806535No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3870.44810337640683623No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3770.43652447779167247No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3710.4295771386225742No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3710.4295771386225742No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3640.4214719095919596No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3430.3971562225001158No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3420.3959983326385994No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3380.3913667731925339No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3310.3832615441619193No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3070.35547218748552634No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3030.3508406280394609No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3020.3496827381779445No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2950.3415775091473299No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2920.3381038395627808No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2890.33463016997823164No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2840.3288407206706498No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2740.3172618220554861No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2730.3161039321939697No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2580.29873558427122415No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2540.2941040248251586No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2540.2941040248251586No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2510.2906303552406095No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2500.28947246537909316No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2490.2883145755175768No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2460.28484090593302763No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2330.2697883377333148No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2330.2697883377333148No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2290.2651567782872493No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2280.26399888842573294No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2090.24199898105692186No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2090.24199898105692186No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2080.2408410911954055No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2070.23968320133388915No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2060.23852531147237274No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2030.23505164188782363No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2030.23505164188782363No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2020.23389375202630727No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1980.22926219258024177No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1980.22926219258024177No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1960.226946412857209No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1930.2234727432726599No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1910.22115696354962716No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1880.21768329396507805No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1850.21420962438052893No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1800.20842017507294708No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1780.20610439534991432No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1730.20031494604233246No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1700.19684127645778335No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1700.19684127645778335No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1690.19568338659626694No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1680.19452549673475059No Hit
GGACTACAGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1670.1933676068732342No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1640.18989393728868512No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1620.18757815756565235No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1600.18526237784261962No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1580.18294659811958686No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1540.1783150386735214No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1540.1783150386735214No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1520.17599925895048862No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1500.1736834792274559No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1480.17136769950442313No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1480.17136769950442313No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1460.1690519197813904No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1440.16673614005835766No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1440.16673614005835766No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1430.16557825019684128No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1390.16094669075077578No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1380.15978880088925942No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1350.1563151313047103No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1350.1563151313047103No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1340.15515724144319393No Hit
GGACTACAAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1330.15399935158167755No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1300.15052568199712843No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1300.15052568199712843No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1280.1482099022740957No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1270.1470520124125793No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1270.1470520124125793No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1260.14589412255106293No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1250.14473623268954658No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1220.14126256310499744No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1190.13778889352044835No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1180.13663100365893197No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1170.13547311379741558No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1170.13547311379741558No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1160.13431522393589923No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1150.13315733407438285No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1150.13315733407438285No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1130.1308415543513501No Hit
GGACTACCGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1130.1308415543513501No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1130.1308415543513501No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1120.12968366448983373No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1110.12852577462831735No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1110.12852577462831735No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1100.127367884766801No Hit
GGACTACACGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1100.127367884766801No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1090.12620999490528462No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1060.1227363253207355No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1050.12157843545921912No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1050.12157843545921912No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1030.11926265573618637No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1030.11926265573618637No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGACATAATCTCATT1020.11810476587466999No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC990.11463109629012089No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC980.1134732064286045No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC960.11115742670557177No Hit
GGACTACCGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA960.11115742670557177No Hit
GGACTACTGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC960.11115742670557177No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC950.1099995368440554No Hit
GGACTACAAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG920.10652586725950627No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC920.10652586725950627No Hit
GGACTACTCGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG920.10652586725950627No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC920.10652586725950627No Hit
GGACTACAAGGGTTTCTAATGGTCCCTGGAGTTTCTCGGCACAGAGCTCA920.10652586725950627No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC900.10421008753647354No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC880.10189430781344078No Hit
GGACTACTAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG880.10189430781344078No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC870.10073641795192441No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA870.10073641795192441No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGACATAATCTCATT870.10073641795192441No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGC108.451498E-4295.00003295
CCAACGG108.451498E-4295.00003295
GGCCTAC108.451498E-4295.000031
CCGGGTA2900.0295.08
GGACTAC80700.0294.451661
TACCGGG6100.0292.581975
ACCCGGG6000.0292.54176
GGGGTAT10550.0292.20389
GACTACC20050.0292.057372
ACACGGG9600.0291.927066
GGGGTTT11600.0291.185339
TACTCGG10600.0290.82555
CTCGGGT10550.0290.80577
CGGGTAT12550.0290.298839
ACCGGGG6150.0290.203256
CACGGGT9700.0288.917547
TACTGGG8850.0288.33335
ACTGGGG8850.0288.33336
ACAAGGG13250.0288.320776
ACTACCG6250.0287.923
Produced by FastQC (version 0.11.5)