FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77895
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA19602.5162077155144744No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA17932.301816547917068No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14671.8833044482957826No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14151.8165479170678476No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13511.7343860324796199No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA13101.6817510751652867No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA13031.672764619038449No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12991.6676295012516849No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA12021.5431028949226524No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA11831.5187110854355221No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11561.4840490403748636No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA11461.4712112459079532No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10391.3338468451120098No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA10171.3056036972848064No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9811.2593876372039283No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9801.2581038577572372No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9751.251684960523782No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9341.1990500032094487No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8981.1528339431285706No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8941.1476988253418063No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8921.1451312664484241No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8751.1233070158546763No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8541.096347647474164No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8341.0706720585403429No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7881.011618203992554No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7861.009050645099172No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7650.9820912767186597No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7300.9371589960844726No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6580.8447268759227164No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5910.7587136529944156No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5870.7535785352076514No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5690.7304705051672123No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5320.682970665639643No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5080.6521599589190576No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5050.6483086205789845No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4960.636754605558765No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4930.6329032672186918No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4760.6110790166249438No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4610.591822324924578No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4420.5674305154374478No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4150.5327684703767893No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4080.5237820142499519No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3940.505809101996277No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3910.5019577636562038No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3890.4993902047628217No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3670.4711470569356184No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3620.46472815970216313No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3600.462160600808781No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3590.46087682136208996No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3410.4377687913216509No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3380.43391745298157774No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3160.40567430515437447No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3140.40310674626099235No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3080.395404069580846No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3080.395404069580846No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2940.3774311573271712No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2830.36330958341356956No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2800.3594582450734964No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2800.3594582450734964No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2680.34405289171320363No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2660.3414853328198215No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA2620.3363502150330573No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2510.32222864111945565No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2510.32222864111945565No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2500.3209448616727646No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2340.30040439052570767No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2340.30040439052570767No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2240.2875665960587971No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2110.27087746325181333No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2080.26702612491174016No Hit
GGACTACAAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA2060.26445856601835804No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2040.2618910071249759No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1940.24905321265806532No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1910.24520187431799215No Hit
GGACTACTCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1900.2439180948713011No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1890.24263431542461003No Hit
GGACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1860.23878297708453686No Hit
GGACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1830.23493163874446368No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1810.23236407985108157No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1800.2310803004043905No Hit
GGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1750.22466140317093522No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.2220938442775531No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1720.22081006483086205No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1690.21695872649078887No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1680.21567494704409781No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1650.21182360870402464No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1580.20283715257718724No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1570.20155337313049618No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1550.19898581423711406No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1540.197702034790423No Hit
GGACTACACGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1490.19128313755696771No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1480.18999935811027666No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1450.18614801977020348No Hit
GGACTACCGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1410.18101290198343925No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1410.18101290198343925No Hit
GGACTACCCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1390.17844534309005713No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1340.17202644585660182No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1320.1694588869632197No Hit
GGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1280.16432376917645547No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1250.1604724308363823No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1240.15918865138969124No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1230.15790487194300018No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1220.15662109249630912No Hit
GGACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1200.154053533602927No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1190.15276975415623595No Hit
GGACTACCAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1130.1450670774760896No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1130.1450670774760896No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1120.14378329802939854No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1110.14249951858270749No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1090.13993195968932537No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1060.1360806213492522No Hit
GGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1030.13222928300917902No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1010.1296617241157969No Hit
GGACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1000.12837794466910585No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1000.12837794466910585No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG970.12452660632903266No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA960.1232428268823416No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG930.11939148854226843No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC900.11554015020219525No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG890.1142563707555042No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG880.11297259130881314No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA860.11040503241543102No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC860.11040503241543102No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA840.10783747352204891No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA800.10270235573528468No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA780.10013479684190256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAC74950.0295.01
ACGGGTA2300.0295.08
GTTAACC157.214516E-6295.0295
CTACCTG108.449902E-4295.04
TACTCCG108.449902E-4295.05
AGTTAGG303.6379788E-12295.0295
TACTCGG12850.0294.999975
GACTACT34500.0294.144932
CTCGGGT12900.0293.85667
CAAGGGT11750.0293.74477
ACCCGGG8400.0293.244026
TCGGGTT6950.0292.87778
ACTACCG6850.0292.84673
CTACTCG13000.0292.730774
ACTCGGG12900.0292.713176
AAGGGTA6000.0292.541668
AGGGTAT11950.0292.53149
TACAAGG11900.0292.521035
GACTACC20900.0291.471282
TACTAGG12300.0291.402445
Produced by FastQC (version 0.11.5)