FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60704
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT610.10048761201897734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA1150.0294.994262
TCGACCT350.0294.994261
GCGACCT206.137634E-8294.994231
CGCGCCT157.207256E-6294.994231
CGACCCT255.2568794E-10294.994231
GCGCCTA500.0294.994232
GCGCCCT303.6379788E-12294.994231
ACGGGTA400.0294.994238
TCGGAAA108.445817E-4294.99423295
TACGCCT108.445817E-4294.994231
CTACGAG206.137634E-8294.994236
CTCGCCT206.137634E-8294.994231
GCTACCT255.2568794E-10294.994231
CGTCCTA1200.0294.994232
CGTCCCT108.445817E-4294.994231
TTACCCT157.207256E-6294.994231
CGATCCT206.137634E-8294.994231
GCGTCCT255.2568794E-10294.994231
AGTACCT255.2568794E-10294.994231
GAGCCTA650.0294.99422
Produced by FastQC (version 0.11.5)