FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76027
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1250.0295.000032
TCTGCCT303.6379788E-12295.01
TTGGCCT550.0295.01
GTCGCCT255.2568794E-10295.01
ACGCCTA1200.0295.02
ACGCCCT255.2568794E-10295.01
CCCTCCT350.0295.01
TGGACCT303.6379788E-12295.01
ATTACCT303.6379788E-12295.01
CGCGCCT255.2568794E-10295.01
GCGCCCT303.6379788E-12295.01
GACCCTA900.0295.02
CCGTCCT600.0295.01
GCACCCT255.2568794E-10295.01
GTTACCT500.0295.01
TACGCCT255.2568794E-10295.01
CGCTCCT255.2568794E-10295.01
ACAGCCT255.2568794E-10295.01
GTTCCCT450.0295.01
CTACCTA1350.0295.02
Produced by FastQC (version 0.11.5)