FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63138
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC920.14571256612499606No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.13304190820108336No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC830.13145807596059425No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC830.13145807596059425No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC800.12670657923912698No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.1251227469986379No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.1251227469986379No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.1251227469986379No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC780.12353891475814882No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.12037125027717065No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.11878741803668155No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.11878741803668155No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.11878741803668155No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.11720358579619247No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.11720358579619247No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.11720358579619247No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.11561975355570336No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.11561975355570336No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.11561975355570336No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.11561975355570336No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.1140359213152143No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.1140359213152143No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC700.11086825683423612No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.10928442459374704No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.10928442459374704No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.10770059235325795No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.10770059235325795No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.10770059235325795No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.10611676011276887No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.10611676011276887No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC660.10453292787227977No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.10453292787227977No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.10453292787227977No Hit
GGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.10453292787227977No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.10453292787227977No Hit
ATGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.10453292787227977No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.10453292787227977No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.1029490956317907No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1029490956317907No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.1029490956317907No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.1029490956317907No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.10136526339130159No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.10136526339130159No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.10136526339130159No Hit
CGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.10136526339130159No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.10136526339130159No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.10136526339130159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1000.0295.02
GCCCCCT108.4461126E-4295.01
GTCGCCT255.2568794E-10295.01
CCCCTAC3750.0295.03
TGCCCCT350.0295.01
AGATCCT303.6379788E-12295.01
ACGCCTA850.0295.02
ACGCCCT255.2568794E-10295.01
GCGACCT303.6379788E-12295.01
CTACTGG157.208044E-6295.06
TCACCCT206.138907E-8295.01
CACGGGA350.0295.06
CTATCCT206.138907E-8295.01
AGCCCTA800.0295.02
CTCCCTA800.0295.02
GGTCCTA850.0295.02
GGTCCCT255.2568794E-10295.01
CGACCTA1050.0295.02
AAGACCT157.208044E-6295.01
CACGCCT206.138907E-8295.01
Produced by FastQC (version 0.11.5)