FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61099
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.13748179184602038No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.13093503985335275No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1243882878606851No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.1194782238661844No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11784153586801749No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11456815987168367No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11456815987168367No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11293147187351675No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11293147187351675No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11129478387534986No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11129478387534986No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10965809587718293No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10965809587718293No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.10802140787901603No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.1063847198808491No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10311134388451529No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10311134388451529No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10311134388451529No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1014746558863484No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1014746558863484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCT350.0295.000031
CGATCCT350.0295.000031
CGGCGGC108.4454415E-4295.09
ACGCCTA800.0295.02
GCGACCT255.2568794E-10295.01
TAAAGCC108.4454415E-4295.0295
CTATCCT550.0295.01
ATAGCCT206.13727E-8295.01
CGCGCCT108.4454415E-4295.01
GCGCCCT400.0295.01
TACGGCG108.4454415E-4295.07
TCCTAGG255.2568794E-10295.04
TACGCCT450.0295.01
ACGGCGG108.4454415E-4295.08
CTACGAG206.13727E-8295.06
CCGACGG108.4454415E-4295.05
CCGACCT206.13727E-8295.01
CGTCCTA900.0295.02
ATCGCCT206.13727E-8295.01
GTGCCTA206.13727E-8295.02
Produced by FastQC (version 0.11.5)