FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69669
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.10478117957771749No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10334582095336521No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10047510370466062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTAC4150.0295.000033
GACCTAC3850.0295.000033
GCCCCTA1050.0295.02
GTACCTA700.0295.02
GTCGCCT450.0295.01
GTTTCCT255.2568794E-10295.01
ATGGCCT255.2568794E-10295.01
AGCCCTA550.0295.02
TAGACCT255.2568794E-10295.01
GGTCCTA1150.0295.02
TTGTCCT350.0295.01
GGTCCCT255.2568794E-10295.01
GACCCTA650.0295.02
CGCTCCT255.2568794E-10295.01
CTTGCCT255.2568794E-10295.01
AGCGCCT350.0295.01
GGAACCT450.0295.01
CCCCCTA850.0295.02
CCGACCT350.0295.01
ACCACCT350.0295.01
Produced by FastQC (version 0.11.5)