FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92762
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCCT550.0294.996251
ACCTCGG350.0294.996254
CGAACCT157.2193343E-6294.996221
ACGCCTA900.0294.996222
CAACGGG108.4528327E-4294.996226
AGCCCTA900.0294.996222
GATCCTA1600.0294.996222
ATTACCT303.6379788E-12294.996221
TTGTCCT303.6379788E-12294.996221
CGCGCCT255.275069E-10294.996221
CCTACGA157.2193343E-6294.996225
GCGCCCT303.6379788E-12294.996221
TACGCCT255.275069E-10294.996221
CGCTCCT303.6379788E-12294.996221
TACGAGG157.2193343E-6294.996227
CTGCCTA450.0294.996222
TCCACGG255.275069E-10294.996224
TTGCCTA750.0294.996222
TTGCCCT400.0294.996221
GGCGCCT255.275069E-10294.996221
Produced by FastQC (version 0.11.5)