FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78769
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTA950.0295.000032
TGATCCT108.4500876E-4295.01
TCGCCTC108.4500876E-4295.02
GCCCCTA900.0295.02
GATACCT303.6379788E-12295.01
ATCTCCT450.0295.01
GTCGCCT255.275069E-10295.01
GCCCACG108.4500876E-4295.03
TGCCCCT206.148548E-8295.01
AGATCCT350.0295.01
CGAACCT350.0295.01
ACGCCTA1100.0295.02
TCACCCT500.0295.01
CAACGGG108.4500876E-4295.06
CTTCCCT157.2148323E-6295.01
CAGCCTA750.0295.02
CCTAGGG350.0295.05
CGGCCTA600.0295.02
GAGTCGG108.4500876E-4295.0295
CCCTAGG108.4500876E-4295.04
Produced by FastQC (version 0.11.5)