FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99465
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT450.0295.01
CCTTCCT550.0295.01
GTGTTGG108.453425E-4295.0295
AATCCTA1850.0295.02
AGTCCTA1500.0295.02
GATACCT450.0295.01
ATCTCCT400.0295.01
GTCGCCT206.1567334E-8295.01
TGCCCCT255.275069E-10295.01
AGATCCT350.0295.01
CGAACCT108.453425E-4295.01
TCACCCT350.0295.01
CAACGGG206.1567334E-8295.06
CTTCCCT350.0295.01
CATGCCT255.275069E-10295.01
CGGCCTA850.0295.02
CTATCCT900.0295.01
TGGACCT305.456968E-12295.01
TCTTCCT800.0295.01
ACACCCT400.0295.01
Produced by FastQC (version 0.11.5)