FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64951
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCT350.0295.000031
TGATCCT450.0295.01
TCGCCTA600.0295.02
GCCCCCT157.209017E-6295.01
CGCGCCT108.4466825E-4295.01
GCGCCTA600.0295.02
CCTACGA157.209017E-6295.05
GCGCCCT255.2568794E-10295.01
ACCTCGG108.4466825E-4295.04
CGCCTCG108.4466825E-4295.03
CCGACCT157.209017E-6295.01
GACGCCT206.1403625E-8295.01
GGCGCCT206.1403625E-8295.01
GCTACGG108.4466825E-4295.04
GATCTAG108.4466825E-4295.0295
CGTCCTA950.0295.02
GATTCCT206.1403625E-8295.01
GCGTCCT157.209017E-6295.01
ATCGCCT108.4466825E-4295.01
GTGCCTA550.0295.02
Produced by FastQC (version 0.11.5)