FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68152
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC910.13352506162695152No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.1291231365183707No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.12472121140978988No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.12325390304026293No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.12325390304026293No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.12325390304026293No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.11885197793168212No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.11591736119262823No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.1144500528231013No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11298274445357437No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11298274445357437No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11298274445357437No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11298274445357437No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.11151543608404742No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.11004812771452047No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.11004812771452047No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10858081934499356No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10858081934499356No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10858081934499356No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1071135109754666No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1071135109754666No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1071135109754666No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1071135109754666No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1071135109754666No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10564620260593965No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10417889423641272No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10417889423641272No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10417889423641272No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10417889423641272No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC700.1027115858668858No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC700.1027115858668858No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.10124427749735884No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.10124427749735884No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.10124427749735884No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.10124427749735884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA950.0295.000032
CATTCCT550.0295.000031
GTGCTGG550.0295.00003295
TTGCCTA950.0295.000032
GAACCTA950.0295.000032
GTGCCTA550.0295.000032
AGTCCTA1000.0295.02
AGCAAAT255.2568794E-10295.0295
GTCGCCT303.6379788E-12295.01
AGTCCCT206.142545E-8295.01
CGAACCT350.0295.01
CACGGGA303.6379788E-12295.06
AGGAGGC108.4475946E-4295.08
CCTAGGA108.4475946E-4295.05
GAGTCCT157.210576E-6295.01
GGGTCCT350.0295.01
ACACCTA1550.0295.02
GCAACTA108.4475946E-4295.01
GTCCTAC4050.0295.03
GATCCTA750.0295.02
Produced by FastQC (version 0.11.5)