FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76070
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCTA950.0295.000032
TGGACCT350.0295.000031
GGTCCTA950.0295.000032
TGAACCT350.0295.000031
TAGCCTA850.0295.000032
AGAACCT350.0295.000031
CGCTCCT350.0295.000031
TATTCCT350.0295.000031
GTCACCT550.0295.000031
GTTCCCT550.0295.000031
GGGCCTA700.0295.000032
TTGCCCT350.0295.000031
GAACCTA950.0295.000032
TACACCT700.0295.000031
CCACCCT350.0295.000031
TGATCCT157.213861E-6295.01
CCTTCCT450.0295.01
GCCCCTA900.0295.02
AGTCCTA1000.0295.02
AGCAAAT108.449519E-4295.0295
Produced by FastQC (version 0.11.5)