FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73700
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA1450.0295.02
AGCCCTA900.0295.02
CAGCCCT255.2568794E-10295.01
ATTACCT255.2568794E-10295.01
CTCCCTA1050.0295.02
GGTCCTA1150.0295.02
GGATCCT450.0295.01
GCGCCTA1050.0295.02
GCACCCT350.0295.01
AAGCCTA1100.0295.02
AATGCCT255.2568794E-10295.01
CTGCCTA1050.0295.02
GGGCCTA750.0295.02
GGGCCCT255.2568794E-10295.01
GACGCCT255.2568794E-10295.01
TGGGCCT350.0295.01
GGACCCT255.2568794E-10295.01
ACTCCCT350.0295.01
ACATCCT550.0295.01
GACCTAC4600.0295.03
Produced by FastQC (version 0.11.5)