FastQCFastQC Report
Wed 8 May 2024
EGAF00005491135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66963
Total Bases20.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.11498887445305617No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10752206442363693No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10453534041186925No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.1000552543942177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)