FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72957
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1260.17270446975615775No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.1576270954123662No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.15351508422769577No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1060.14529106185835491No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.14392039146346478No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.14392039146346478No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.1411790506736845No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.13980838027879436No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.13980838027879436No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.13980838027879436No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.13843770988390422No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.13843770988390422No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.13843770988390422No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.13706703948901408No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.13706703948901408No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.13706703948901408No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.1343256986992338No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.13295502830434366No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.1315843579094535No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12884301711967322No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12884301711967322No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12884301711967322No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12884301711967322No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12747234672478308No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.12747234672478308No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.12610167632989294No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.12610167632989294No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.12336033554011266No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.12336033554011266No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.12336033554011266No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.12198966514522253No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.12198966514522253No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.12198966514522253No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.12198966514522253No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12061899475033239No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12061899475033239No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12061899475033239No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12061899475033239No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12061899475033239No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11924832435544225No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11924832435544225No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11787765396055211No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11787765396055211No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11787765396055211No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11650698356566196No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11650698356566196No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11650698356566196No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11650698356566196No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11650698356566196No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.11513631317077182No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.11513631317077182No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.11513631317077182No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.11513631317077182No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.11513631317077182No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.11513631317077182No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11376564277588168No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11376564277588168No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11376564277588168No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11376564277588168No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11376564277588168No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11239497238099154No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11239497238099154No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11239497238099154No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11239497238099154No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11239497238099154No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11239497238099154No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11239497238099154No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11239497238099154No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11239497238099154No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1110243019861014No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1110243019861014No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1110243019861014No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1110243019861014No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10965363159121126No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10965363159121126No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10965363159121126No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10965363159121126No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10828296119632112No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10828296119632112No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10691229080143098No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10691229080143098No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10691229080143098No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10691229080143098No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10691229080143098No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10691229080143098No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10554162040654085No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10554162040654085No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10554162040654085No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10554162040654085No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10554162040654085No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.1041709500116507No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.1041709500116507No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.1041709500116507No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.1041709500116507No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.1041709500116507No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10280027961676057No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10280027961676057No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10280027961676057No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10280027961676057No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10280027961676057No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10280027961676057No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10280027961676057No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1014296092218704No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1014296092218704No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1014296092218704No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1014296092218704No Hit
TATGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1014296092218704No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10005893882698026No Hit
GAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10005893882698026No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10005893882698026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)