FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74773
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.1176895403421021No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11234001578109745No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.1110026346408463No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.10966525350059513No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10966525350059513No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.10832787236034398No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10699049122009281No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.10431572893959049No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10164096665908817No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.10164096665908817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCT255.2386895E-10295.195471
ACGCCTA950.0295.195472
TGCACCT400.0295.195471
GCCTCCT108.432476E-4295.195471
GTCACCT400.0295.195471
CCTACCT255.2386895E-10295.195471
TTACCCT400.0295.195471
GGACCTA1050.0295.195472
ACGTCCT350.0295.195471
AGGACCT255.2386895E-10295.195471
CCTGCCT108.432476E-4295.195471
GGCGCCT108.432476E-4295.195471
CACGCCT255.2386895E-10295.195471
GTTCCCT400.0295.195471
TATGCCT350.0295.195471
TCGGCCT400.0295.195471
TAGCCTA1050.0295.195472
TGATCCT255.2386895E-10295.195471
CTCTCCT350.0295.195471
CGCTCCT108.432476E-4295.195471
Produced by FastQC (version 0.11.5)