FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284082
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC3480.12249984159503242No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC3480.12249984159503242No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC3240.11405157665744398No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC3190.11229152146211305No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC3130.11017945522771594No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC3110.10947543314958356No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC3090.1087714110714512No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC3050.10736336691518646No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC2990.10525130068078936No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC2980.10489928964172315No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC2980.10489928964172315No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC2960.1041952675635908No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC2960.1041952675635908No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC2930.10313923444639224No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC2910.10243521236825988No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC2890.10173119029012749No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC2870.10102716821199513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT700.0295.01
CGAACCT1200.0295.01
CGCGCCT750.0295.01
CCTACGA305.456968E-12295.05
GTCTACG157.2346356E-6295.03
GACCCCT1700.0295.01
GCACCCT1300.0295.01
TACGCCT1050.0295.01
CTACCGG157.2346356E-6295.06
CCGACCT900.0295.01
GACGCCT1000.0295.01
CGCACCT950.0295.01
TTACCAC157.2346356E-6295.02
CCGCCCT650.0295.01
ACGACCT950.0295.01
CGACCTC108.461679E-4294.999972
TAGCCCT800.0294.999971
CGCCTAG108.461679E-4294.999973
ACTAGCG108.461679E-4294.99997295
TACGCAC108.461679E-4294.99997295
Produced by FastQC (version 0.11.5)