FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90438
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.10062142019947368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCTA1700.0295.000032
TCGCCTA1300.0295.02
TCTGCCT500.0295.01
TTGGCCT206.153641E-8295.01
GTCGCCT350.0295.01
AGATCCT303.6379788E-12295.01
CGAACCT206.153641E-8295.01
GCGACCT157.218361E-6295.01
TTAGCCT350.0295.01
GATCCTA1500.0295.02
GATCCCT255.275069E-10295.01
GGTCCCT157.218361E-6295.01
CGCGCCT303.6379788E-12295.01
GCGCCTA1000.0295.02
CCTACGA157.218361E-6295.05
TAGCCTA1150.0295.02
ATATCCT108.4521546E-4295.01
TGCGCCT206.153641E-8295.01
CTACGAG157.218361E-6295.06
ATCCCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)