FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72973
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.13292587669411973No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12470365751716388No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.1233332876543379No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.12059254792868596No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.117851808203034No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11511106847738205No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.1123703287517301No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10962958902607813No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10414810957477423No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10277773971194826No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10277773971194826No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10277773971194826No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10277773971194826No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10277773971194826No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10140736984912228No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10140736984912228No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10140736984912228No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10140736984912228No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1000369999862963No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1000369999862963No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.1000369999862963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCT550.0295.000031
GTCCTAC4400.0295.000033
AGGCCTA1100.0295.000032
TCTCCTA1700.0295.000032
CTACCTA850.0295.000032
TCCCTAC3400.0295.000033
GCCCTAG108.4488146E-4295.03
ATCCCCT255.2568794E-10295.01
GTTTGTC255.2568794E-10295.0295
TTGTCCT400.0295.01
TGGTCCT206.145456E-8295.01
TGCACCT206.145456E-8295.01
GGGTCCT206.145456E-8295.01
TCCCACG108.4488146E-4295.03
GCCTCCT206.145456E-8295.01
CCTACGA157.212657E-6295.05
CCTACCT157.212657E-6295.01
ATCTCCT303.6379788E-12295.01
CACACCT350.0295.01
TGACCTA1000.0295.02
Produced by FastQC (version 0.11.5)