FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71228
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.14179816925928007No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.13056663110012917No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.12775874656034142No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12354691975065984No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11933509294097826No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.1179311506710844No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11652720840119055No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11231538159150896No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11231538159150896No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11231538159150896No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10950749705172123No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10950749705172123No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10810355478182737No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10810355478182737No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10810355478182737No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.1066996125119335No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10529567024203965No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10529567024203965No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10389172797214578No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10389172797214578No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10248778570225191No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10248778570225191No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10248778570225191No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10108384343235806No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10108384343235806No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10108384343235806No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10108384343235806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCG108.4483885E-4295.06
GCCCCTA550.0295.02
GCCCCCT206.144546E-8295.01
TTCTACG108.4483885E-4295.03
ACGCCTA950.0295.02
GCGACCT255.2568794E-10295.01
CGTGCCT206.144546E-8295.01
CCGGCGG108.4483885E-4295.07
ATGGACA108.4483885E-4295.0295
GATCCTA750.0295.02
TTGTCCT350.0295.01
GATCCCT255.2568794E-10295.01
CGACCTA1100.0295.02
CACGCCT255.2568794E-10295.01
GCGCCTA900.0295.02
GCGCCCT206.144546E-8295.01
ACGGGGC255.2568794E-10295.08
GTTACCT500.0295.01
TTCCCTA1450.0295.02
CGGGGCA255.2568794E-10295.09
Produced by FastQC (version 0.11.5)