FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69826
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1180.1689914931400911No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1140.16326296794890158No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1130.16183083665110418No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1060.1518059175665225No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.1503737862687251No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.1503737862687251No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.14894165497092773No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.14894165497092773No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.14750952367313033No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.14750952367313033No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.146077392375333No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.146077392375333No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.1446452610775356No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.1432131297797382No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.1432131297797382No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.1417809984819408No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.14034886718414347No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.14034886718414347No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.14034886718414347No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.13891673588634607No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.13891673588634607No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.13748460458854866No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.1360524732907513No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.1360524732907513No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.1360524732907513No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.1360524732907513No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.13462034199295392No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.13462034199295392No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.13462034199295392No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.13462034199295392No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.13318821069515654No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.13318821069515654No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.13318821069515654No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.13318821069515654No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.13318821069515654No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.13318821069515654No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.13175607939735914No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.13175607939735914No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.13175607939735914No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.13032394809956177No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.13032394809956177No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.1288918168017644No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.127459685503967No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.127459685503967No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12602755420616962No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.12602755420616962No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.12459542290837224No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.12316329161057486No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.12173116031277748No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.1202990290149801No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.1202990290149801No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.1202990290149801No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.1202990290149801No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.1202990290149801No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11886689771718272No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11886689771718272No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11886689771718272No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11886689771718272No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11886689771718272No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11886689771718272No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11886689771718272No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11886689771718272No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11743476641938531No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11743476641938531No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11743476641938531No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11743476641938531No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11743476641938531No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11600263512158795No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11600263512158795No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11600263512158795No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11600263512158795No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11600263512158795No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11457050382379055No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11457050382379055No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11457050382379055No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11457050382379055No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11457050382379055No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.1131383725259932No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.1131383725259932No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.1131383725259932No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.1117062412281958No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.1117062412281958No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.1117062412281958No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.1117062412281958No Hit
GACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.1117062412281958No Hit
ACCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.1117062412281958No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11027410993039843No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11027410993039843No Hit
TGTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11027410993039843No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11027410993039843No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11027410993039843No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11027410993039843No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11027410993039843No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10884197863260103No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10884197863260103No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10884197863260103No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10884197863260103No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10884197863260103No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10884197863260103No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10740984733480365No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10740984733480365No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10740984733480365No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10740984733480365No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10740984733480365No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10597771603700627No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10597771603700627No Hit
CAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10597771603700627No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10597771603700627No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10597771603700627No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10597771603700627No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10597771603700627No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10597771603700627No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10454558473920889No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10454558473920889No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10454558473920889No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10454558473920889No Hit
GTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10454558473920889No Hit
GTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10454558473920889No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10311345344141151No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10311345344141151No Hit
GGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10311345344141151No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10311345344141151No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10311345344141151No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10311345344141151No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10168132214361413No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10168132214361413No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10168132214361413No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10168132214361413No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10168132214361413No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10168132214361413No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10168132214361413No Hit
GCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
ACAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
AAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
AGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
ACGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
CCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
TGGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit
CGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10024919084581675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)