FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71549
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.11460677298075445No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.10761855511607431No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10622091154313827No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.1034256243972662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAC3850.0295.000033
CCACCTA1100.0295.000032
TGATCCT500.0295.01
TCGCCCT350.0295.01
GCCCCCT255.2568794E-10295.01
GTCGCCT303.6379788E-12295.01
TGCCCCT400.0295.01
CGAACCT303.6379788E-12295.01
GCGACCT157.2120565E-6295.01
ATCTACG108.448463E-4295.03
CAACGGG108.448463E-4295.06
CTATCCT303.6379788E-12295.01
CGTGCCT303.6379788E-12295.01
ACCGCCT255.2568794E-10295.01
CTCCCTA450.0295.02
GGTCCTA1200.0295.02
TCCCTAC3300.0295.03
CGCGCCT157.2120565E-6295.01
GCGCCTA1000.0295.02
CCTACGA108.448463E-4295.05
Produced by FastQC (version 0.11.5)