FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101566
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCT550.0295.000031
GCCCCTA1350.0295.02
GTCGCCT255.275069E-10295.01
GAGCCTA1000.0295.02
CGTACCT255.275069E-10295.01
CGATCCT350.0295.01
ATCGCCT350.0295.01
CCCGCCT450.0295.01
ATCCTCG108.453689E-4294.999973
TGGACCT400.0294.999971
TTGTCCT600.0294.999971
CGTTCCT400.0294.999971
GCCGCCT305.456968E-12294.999971
CTGCGGG108.453689E-4294.999976
CTAACGG108.453689E-4294.999976
ACCTAGG157.2209823E-6294.999974
TACGCCT400.0294.999971
CGCTCCT400.0294.999971
TACCTAG108.453689E-4294.999973
CTCGCCT206.157279E-8294.999971
Produced by FastQC (version 0.11.5)