FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76408
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.12040624018427389No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.10208355146058004No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.1007747879803162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT350.0295.000031
TCGCCTA700.0295.000032
TGAACCT350.0295.000031
GGTCATT350.0295.00003295
TAGCCTA700.0295.000032
CGCTCCT350.0295.000031
GATTCCT350.0295.000031
CACCCTA1400.0295.000032
GTGCCTA700.0295.000032
GCCCCTA1150.0295.02
GCCCCCT255.2568794E-10295.01
TGCCCCT303.6379788E-12295.01
ACGCCTA1250.0295.02
TCACCCT450.0295.01
CAACGGG108.4495946E-4295.06
CCTAGGG600.0295.05
CGGCCTA1000.0295.02
CGTGCCT303.6379788E-12295.01
GAGTCCT303.6379788E-12295.01
CCGGCCT400.0295.01
Produced by FastQC (version 0.11.5)