FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91023
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.10217197851092581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT450.0295.01
GCCCCTA1100.0295.02
AATCCTA1800.0295.02
ATCTCCT255.275069E-10295.01
GTCGCCT206.153823E-8295.01
AGCCCTA1400.0295.02
GATCCTA1800.0295.02
AAGACCT255.275069E-10295.01
TGAACCT157.2185176E-6295.01
GCGCCTA950.0295.02
CCTACGA157.2185176E-6295.05
GCGCCCT255.275069E-10295.01
CGTTCCT255.275069E-10295.01
AACGGGA108.452246E-4295.07
TACGCCT303.6379788E-12295.01
TATTCCT350.0295.01
GTCACCT255.275069E-10295.01
CCCCCTA1100.0295.02
CTACCTA1400.0295.02
CATACCT450.0295.01
Produced by FastQC (version 0.11.5)