FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70457
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC1140.16180081468129498No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC1100.15612359311353025No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC1050.14902706615382433No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC1050.14902706615382433No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC1030.14618845536994196No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC1020.14476914997800075No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC1020.14476914997800075No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC1020.14476914997800075No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC990.1405112338021772No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC970.13767262301829483No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC970.13767262301829483No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC960.13625331762635368No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC950.1348340122344125No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC940.13341470684247128No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC930.13199540145053013No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC920.13057609605858891No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC920.13057609605858891No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC920.13057609605858891No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC910.12915679066664773No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC900.12773748527470657No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC900.12773748527470657No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC900.12773748527470657No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC900.12773748527470657No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC900.12773748527470657No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC900.12773748527470657No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC890.12631817988276536No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC880.1248988744908242No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC880.1248988744908242No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC870.12347956909888301No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC870.12347956909888301No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC870.12347956909888301No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC870.12347956909888301No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC860.12206026370694181No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC850.12064095831500064No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC850.12064095831500064No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC850.12064095831500064No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC850.12064095831500064No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC850.12064095831500064No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.11922165292305945No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.11922165292305945No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.11922165292305945No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.11922165292305945No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.11922165292305945No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC830.11780234753111826No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC830.11780234753111826No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC820.11638304213917709No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC820.11638304213917709No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC820.11638304213917709No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC820.11638304213917709No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC820.11638304213917709No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC820.11638304213917709No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC810.1149637367472359No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC810.1149637367472359No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC810.1149637367472359No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC810.1149637367472359No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC800.11354443135529473No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC800.11354443135529473No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.11212512596335353No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.11212512596335353No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.11212512596335353No Hit
GATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.11212512596335353No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.11212512596335353No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.11212512596335353No Hit
GCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC780.11070582057141234No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC780.11070582057141234No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC780.11070582057141234No Hit
GTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC780.11070582057141234No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC780.11070582057141234No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC780.11070582057141234No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC770.10928651517947117No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC770.10928651517947117No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC770.10928651517947117No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC770.10928651517947117No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.10786720978752998No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.10786720978752998No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.10786720978752998No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
GAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.10644790439558881No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.10502859900364761No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.10502859900364761No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.10502859900364761No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.10502859900364761No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.10502859900364761No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.10502859900364761No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.10502859900364761No Hit
TGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.10360929361170643No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.10360929361170643No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.10360929361170643No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.10360929361170643No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.10218998821976526No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.10218998821976526No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.10218998821976526No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.10218998821976526No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.10218998821976526No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.10077068282782406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)