FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63393
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC800.12619689871121417No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.124619437477324No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC780.12304197624343383No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.11988705377565347No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.11988705377565347No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.11988705377565347No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.11673213130787312No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.11515467007398293No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.11357720884009276No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.11199974760620257No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.10884482513842221No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.10884482513842221No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.10726736390453205No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.10568990267064188No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.10568990267064188No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.10568990267064188No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.10568990267064188No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.10411244143675169No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.10411244143675169No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.10253498020286152No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.10253498020286152No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.10253498020286152No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.10253498020286152No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.10095751896897133No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.10095751896897133No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATT400.0295.0295
GCCCCCT108.446191E-4295.01
ATCCTCG108.446191E-4295.03
AGTCCTA1000.0295.02
AATCCCT255.2568794E-10295.01
TTGGCCT206.139089E-8295.01
GTCGCCT206.139089E-8295.01
GTACCCT108.446191E-4295.01
CCCCTAG108.446191E-4295.03
AGATCCT157.2081784E-6295.01
CGAACCT206.139089E-8295.01
ACGCCCT157.2081784E-6295.01
GTACAGT303.6379788E-12295.0295
GTTTCCT303.6379788E-12295.01
CAGCCTA800.0295.02
CGGCCTA450.0295.02
CGTGCCT255.2568794E-10295.01
AGCCCTA850.0295.02
CCGGCCT108.446191E-4295.01
GTCCTAG108.446191E-4295.03
Produced by FastQC (version 0.11.5)