FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81249
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCT550.0295.000031
ATGGGAT550.0295.00003295
CTATCCT550.0295.000031
GACCCCT350.0295.000031
GCACCCT350.0295.000031
GTAGCCT350.0295.000031
TACGCCT350.0295.000031
TATTCCT550.0295.000031
CCGACCT350.0295.000031
GTCCCTA550.0295.000032
TCCCCCT350.0295.000031
ACATCCT550.0295.000031
CCGCCTA850.0295.000032
GCATCCT350.0295.000031
ATTTCCT350.0295.000031
TGATCCT108.4505713E-4295.01
GTTGATT650.0295.0295
TCTGCCT303.6379788E-12295.01
GCCCCTA1050.0295.02
ACAACCT500.0295.01
Produced by FastQC (version 0.11.5)