FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64063
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA950.0295.000032
TTCCCTA950.0295.000032
GCCCCTA1000.0295.02
GTCGCCT350.0295.01
TGCCCCT255.2568794E-10295.01
ACGCCTA450.0295.02
GGTCCCT157.2085495E-6295.01
CGCGCCT157.2085495E-6295.01
TCCTCGG157.2085495E-6295.04
ACCCTAC3950.0295.03
GACCCCT255.2568794E-10295.01
GCACCTA700.0295.02
AGTGCCT350.0295.01
GGGACCT350.0295.01
CTCGGGA500.0295.06
CTCGCCT500.0295.01
GACGCCT157.2085495E-6295.01
GGACCCT157.2085495E-6295.01
ATGCCCT303.6379788E-12295.01
TAAGCCT157.2085495E-6295.01
Produced by FastQC (version 0.11.5)