FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64063
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA16672.602126032187066No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA14702.2946162371415637No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA12441.9418385027238811No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA10811.687401464183694No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA9871.5406709020807643No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA9091.418915754803865No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA8421.3143312052198617No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA8101.2643803755678005No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA7811.2191124361956198No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA7131.1129669231849897No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA6090.9506267268157907No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA5840.9116026411501178No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA5800.9053587874436102No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA5630.8788224091909526No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA5580.871017592057818No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5420.8460421772317874No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5070.7914084572998454No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4740.7398966642211573No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4190.654043675756677No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4030.6290682609306464No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3990.6228244072241387No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3960.618141516944258No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3710.5791174312785852No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA3560.5557029798791815No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA3510.5478981627460469No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3240.5057521502271202No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3040.4745328816945819No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3020.471410954841328No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2940.4589232474283128No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2750.4292649423224014No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA2740.42770397889577444No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2610.40741145434962456No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA2580.4027285640697438No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2560.39960663721649No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2550.3980456737898631No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2540.3964847103632362No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2490.3886798932301016No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2380.3715092955372056No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2370.36994833211057865No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2260.3527777344176826No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA2250.35121677099105564No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2230.34809484413780184No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2210.344972917284548No Hit
GGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA2080.32468039273839816No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2010.31375364875200973No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1990.3106317218987559No Hit
GGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1950.3043878681922483No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1940.30282690476562135No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1830.28565630707272527No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1790.2794124533662176No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1780.27785148993959075No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1780.27785148993959075No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1780.27785148993959075No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1760.2747295630863369No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1710.2669247459532023No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1710.2669247459532023No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1710.2669247459532023No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1670.26068089224669466No Hit
GGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1630.254437038540187No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1610.2513151116869332No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1610.2513151116869332No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1590.24819318483367933No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1570.24507125798042553No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1500.2341445139940371No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1500.2341445139940371No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1480.2310225871407833No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1470.2294616237141564No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1420.2216568065810218No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.21853487972776797No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1370.21385198944788722No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1370.21385198944788722No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1370.21385198944788722No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1360.21229102602126032No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1350.21073006259463342No Hit
GGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1300.20292524546149884No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1300.20292524546149884No Hit
GGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1290.2013642820348719No Hit
GGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1270.1982423551816181No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1260.1966813917549912No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1250.19512042832836426No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1240.19355946490173737No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1230.19199850147511044No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1230.19199850147511044No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1220.19043753804848354No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1210.1888765746218566No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1180.18419368434197586No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1170.18263272091534893No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1170.18263272091534893No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1150.17951079406209514No Hit
GGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1150.17951079406209514No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1110.17326694035558748No Hit
GGACTACAAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1080.16858405007570673No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1060.16546212322245288No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1060.16546212322245288No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1050.16390115979582598No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1000.15609634266269143No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1000.15609634266269143No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA990.1545353792360645No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA990.1545353792360645No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG980.1529744158094376No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.14985248895618375No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA960.14985248895618375No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA950.14829152552955682No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA950.14829152552955682No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC940.14673056210292992No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA940.14673056210292992No Hit
GGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA910.1420476718230492No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA910.1420476718230492No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA900.14048670839642227No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.14048670839642227No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC890.13892574496979537No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA880.13736478154316845No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA880.13736478154316845No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA870.13580381811654155No Hit
GGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA850.1326818912632877No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA840.13112092783666077No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.13112092783666077No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC820.12799900098340694No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA820.12799900098340694No Hit
GGACTACAAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA820.12799900098340694No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA820.12799900098340694No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA810.12643803755678004No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA810.12643803755678004No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC800.12487707413015314No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.12331611070352622No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA780.12175514727689932No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA780.12175514727689932No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA780.12175514727689932No Hit
GGACTACTAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA780.12175514727689932No Hit
GGACTACTAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA740.11551129357039165No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA740.11551129357039165No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.11395033014376472No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA730.11395033014376472No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA720.11238936671713783No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA720.11238936671713783No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC710.1108284032905109No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA710.1108284032905109No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC710.1108284032905109No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA710.1108284032905109No Hit
GGACTACACGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA710.1108284032905109No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC700.10926743986388399No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA700.10926743986388399No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA690.10770647643725709No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA690.10770647643725709No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA680.10614551301063016No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC680.10614551301063016No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA670.10458454958400326No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA670.10458454958400326No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA670.10458454958400326No Hit
GGACTACTCGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA670.10458454958400326No Hit
GGACTACCAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA660.10302358615737633No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA660.10302358615737633No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA660.10302358615737633No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC660.10302358615737633No Hit
GGACTACAAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG650.10146262273074942No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC650.10146262273074942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTT3000.0295.08
CGGGGTA1400.0295.08
CTCGGGT6250.0295.07
GGGGTAT6150.0295.09
CTACCCG4800.0295.04
AGTTGGC206.139635E-8294.99997295
GGTCTAC108.4464106E-4294.999971
GGACTAC59100.0294.750431
ACACGGG7550.0293.04646
ACAAGGG12000.0292.54176
AGGGTAT15800.0292.199379
CAGGGGT5100.0292.107827
TACTAGG9250.0291.810825
TACAAGG12150.0291.358035
CAGGGTA3850.0291.168858
TACACGG7650.0291.14385
GACTACA24950.0290.861722
CTACCGG3350.0290.5974
ACCGGGG3300.0290.53036
TACCGGG3300.0290.53035
Produced by FastQC (version 0.11.5)