FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102253
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT550.0295.000031
ACGACCT550.0295.000031
GTCGCCT206.157643E-8295.01
TGCCCCT305.456968E-12295.01
CGAACCT500.0295.01
CACGGAG157.221126E-6295.06
CGTGCCT400.0295.01
GTCCTCG108.453773E-4295.03
GATCCTA1600.0295.02
TCTTACG108.453773E-4295.04
AGGACCT350.0295.01
CGCGCCT157.221126E-6295.01
GGATCCT305.456968E-12295.01
CCTACGC206.157643E-8295.05
CCTACGA108.453773E-4295.05
GCGCCCT255.275069E-10295.01
CGCGAGG108.453773E-4295.09
CGTTCCT305.456968E-12295.01
GCACCTA1250.0295.02
GCACCCT305.456968E-12295.01
Produced by FastQC (version 0.11.5)