FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69531
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA18212.618975708676705No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA17152.466525722339676No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA15892.2853115876371692No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA14012.0149285930016827No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13321.9156922811407862No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13121.8869281327753091No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12861.8495347399001882No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12141.7459838057844703No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12091.7387927686931008No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12061.7344781464382792No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12031.7301635241834576No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11931.7157814500007191No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11751.6898937164717895No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11331.6294890049042874No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10211.4684097740576145No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9291.3360946915764191No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9051.3015777135378466No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8721.254116868734809No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6730.9679135924983101No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6320.9089470883490818No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5420.7795084207044339No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5250.7550588945937784No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4830.694654183026276No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4740.6817103162618113No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4740.6817103162618113No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4590.6601372049877033No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4530.6515079604780601No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4460.6414405085501431No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4380.6299348492039522No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4280.6155527750212135No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4010.5767211747278193No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3970.5709683450547237No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3810.547957026362342No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3670.527822122506508No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3630.5220692928334125No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3610.5191928779968648No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3540.5091254260689477No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3520.5062490112324No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3430.49330514446793516No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3410.49042872963138745No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3390.48755231479483974No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3260.4688556183572795No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3180.45734995901108855No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3110.4472825070831715No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2950.4242711883907897No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2930.42139477355424193No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2770.39838345486186016No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2680.3854395880973954No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2640.37968675842429994No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2620.37681034358775223No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2610.3753721361694784No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2610.3753721361694784No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2600.3739339287512045No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2580.3710575139146568No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2580.3710575139146568No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2580.3710575139146568No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2550.3667428916598352No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2550.3667428916598352No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2540.3653046842415613No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2480.3566754397319182No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2480.3566754397319182No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2470.3552372323136443No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2410.3466079878040011No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2410.3466079878040011No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2400.34516978038572727No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2390.3437315729674534No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2340.33654053587608407No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2310.33222591362126247No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2300.3307877062029886No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2230.32072025427507156No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2230.32072025427507156No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2220.3192820468567977No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2220.3192820468567977No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2200.31640563202024996No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2190.3149674246019761No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2140.30777638751060676No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2060.29627072816441585No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2040.29339431332786814No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1980.28476506881822494No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1980.28476506881822494No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1960.28188865398167723No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1950.28045044656340334No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1850.2660683723806648No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1840.2646301649623909No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1810.2603155427075693No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1730.24880988336137838No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1720.24737167594310452No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1680.24161884627000907No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1640.23586601659691364No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1620.23298960176036587No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1600.23011318692381816No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1590.22867497950554427No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1550.22292214983244885No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1510.21716932015935336No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1500.21573111274107953No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1490.21429290532280565No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1460.20997828306798405No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1400.20134903855834085No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1390.19991083114006702No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1380.19847262372179314No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1380.19847262372179314No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1370.19703441630351928No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1290.1855287569573284No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1280.18409054953905454No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1270.18265234212078066No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1260.1812141347025068No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1260.1812141347025068No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1240.1783377198659591No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1230.1768995124476852No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1220.17546130502941135No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1220.17546130502941135No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1220.17546130502941135No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1210.1740230976111375No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1200.17258489019286363No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1120.1610792308466727No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1110.15964102342839884No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1110.15964102342839884No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1090.15676460859185112No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1050.15101177891875567No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA990.14238253440911247No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA980.14094432699083861No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA980.14094432699083861No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA920.13231508248119545No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA920.13231508248119545No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT870.12512404538982613No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA860.12368583797155226No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA840.12080942313500453No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA820.11793300829845682No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA790.11361838604363522No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA790.11361838604363522No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA760.10930376378881362No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA760.10930376378881362No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT720.10355093411571817No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA720.10355093411571817No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT710.10211272669744431No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA700.10067451927917043No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT700.10067451927917043No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)