FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67542
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA22213.288324301915845No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA21943.2483491753279443No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA18712.77012821651713No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA18492.737555891149211No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA14582.1586568357466467No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA14552.1542151550146578No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA14042.078706582570845No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13481.9957952089070505No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA13391.9824701667110836No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA12901.9099227147552633No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA12841.9010393532912853No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12681.877350389387344No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12411.8373752627994435No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11671.727813804743715No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11501.7026442805957775No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10941.619732906931983No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA10851.606407864736016No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10051.4879630452163097No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA10051.4879630452163097No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA9831.4553907198483906No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9631.425779514968464No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA9311.3784015871605815No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8661.2821651713008202No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8471.25403452666489No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA8381.240709484468923No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8281.2259038820289598No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8271.2244233217849634No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8151.2066565988570075No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8021.1874093156850551No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7821.1577981108051285No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7791.1533564300731396No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7751.1474341890971544No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7271.0763672973853304No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7201.0660033756773564No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7041.042314411773415No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6851.014183767137485No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6700.9919753634775399No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6400.9475585561576501No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6230.9223890320097125No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6130.9075834295697492No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5770.8542832607858813No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA5370.7950608510260283No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5360.793580290782032No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5110.7565662846821237No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5060.7491634834621421No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4980.7373190015101714No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA4780.7077077966302449No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4670.6914216339462853No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4590.6795771519943147No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4270.6321992241864322No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3550.5255988866186966No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3010.4456486334428948No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2920.4323235912469278No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2690.39827070563501227No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA2660.3938290249030233No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA2560.37902342246306003No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA2510.3716206212430784No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2300.3405288561191555No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2160.3198010127032069No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA2080.30795653075123625No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1960.29018980782328035No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1960.29018980782328035No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1910.2827870066032987No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1870.2768647656273134No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1640.24281188001539783No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1630.24133131977140151No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1610.23837019928340886No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.22948683781943086No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1530.2265257173314382No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1480.2191229161114566No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1410.20875899440348228No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1380.20431731367149328No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1300.19247283171952265No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.18803115098753367No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1260.18655059074353736No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1200.17766722927955939No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1200.17766722927955939No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1200.17766722927955939No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1180.17470610879156673No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1160.17174498830357407No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1150.17026442805957775No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1130.1673033075715851No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1130.1673033075715851No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1120.16582274732758875No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1110.16434218708359244No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1100.1628616268395961No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA1080.15990050635160344No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1070.15841994610760712No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.15545882561961447No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1030.1524977051316218No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1020.15101714488762547No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1010.14953658464362915No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.14953658464362915No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA990.1465754641556365No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA940.13917266293565483No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA920.1362115424476622No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA920.1362115424476622No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA910.13473098220366586No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA890.1317698617156732No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.1302893014716769No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA880.1302893014716769No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA850.1258476207396879No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA820.12140594000769891No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA800.11844481951970626No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.11696425927570993No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA750.11104201829972463No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA750.11104201829972463No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.1095614580557283No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA720.10660033756773563No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA720.10660033756773563No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACTTTA720.10660033756773563No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA690.10215865683574664No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA690.10215865683574664No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA680.10067809659175032No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA680.10067809659175032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCC350.0295.00003295
GGACTAC65600.0295.000031
TACTCGG7650.0295.000035
CTACTCG7650.0295.000034
CGTTAGA108.4474165E-4295.0295
CGGGGTA1550.0295.08
GGACTAT108.4474165E-4295.01
AATTAGA108.4474165E-4295.0295
ACCCGGG5500.0295.06
AAGGGTA5050.0295.08
TGTTAGC303.6379788E-12295.0295
TACAAGG12250.0295.05
ACTTAGA206.1421815E-8295.0295
ACGGGTA1100.0295.08
TTTAGGC108.4474165E-4295.0295
GTTAGCC1650.0295.0295
AGTTGGC108.4474165E-4295.0295
GGCTACC108.4474165E-4295.01
ACAAGGG12300.0295.06
CAAGGGT12250.0295.07
Produced by FastQC (version 0.11.5)