FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72388
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1280.1768248881030005No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1240.17129911034978174No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1210.16715477703486767No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1160.16024755484334421No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1150.1588661104050395No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1140.15748466596673483No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1140.15748466596673483No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1110.15334033265182073No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1100.15195888821351605No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1090.15057744377521134No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1070.14781455489860199No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1070.14781455489860199No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1070.14781455489860199No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1050.1450516660219926No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1050.1450516660219926No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1050.1450516660219926No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1050.1450516660219926No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1040.1436702215836879No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1030.1422887771453832No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1020.14090733270707853No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1020.14090733270707853No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1010.13952588826877382No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1010.13952588826877382No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1000.13814444383046914No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1000.13814444383046914No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1000.13814444383046914No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT990.13676299939216444No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT990.13676299939216444No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT980.13538155495385976No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT970.13400011051555508No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT960.13261866607725037No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT960.13261866607725037No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT940.12985577720064098No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT940.12985577720064098No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT940.12985577720064098No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT940.12985577720064098No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT940.12985577720064098No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT930.1284743327623363No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT930.1284743327623363No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT930.1284743327623363No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT930.1284743327623363No Hit
CGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT930.1284743327623363No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT920.12709288832403162No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT920.12709288832403162No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT910.12571144388572691No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT910.12571144388572691No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT910.12571144388572691No Hit
ACGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.12432999944742222No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.12432999944742222No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.12432999944742222No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.12432999944742222No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT890.12294855500911753No Hit
ATCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT890.12294855500911753No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.12156711057081285No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.12156711057081285No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.12018566613250815No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.12018566613250815No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.12018566613250815No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.12018566613250815No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.12018566613250815No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.12018566613250815No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.12018566613250815No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.11880422169420347No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.11880422169420347No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.11880422169420347No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.11880422169420347No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.11880422169420347No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.11880422169420347No Hit
GACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.11880422169420347No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.11742277725589877No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11742277725589877No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.11742277725589877No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.11742277725589877No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.11742277725589877No Hit
GTGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.11742277725589877No Hit
TTGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.11742277725589877No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT840.11604133281759407No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.11465988837928939No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.11465988837928939No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.11465988837928939No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.11465988837928939No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT820.1132784439409847No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT820.1132784439409847No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT820.1132784439409847No Hit
CCCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT820.1132784439409847No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11189699950267999No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT810.11189699950267999No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT810.11189699950267999No Hit
ATGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT810.11189699950267999No Hit
AACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT810.11189699950267999No Hit
TTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT810.11189699950267999No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT800.11051555506437531No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT800.11051555506437531No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT800.11051555506437531No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT800.11051555506437531No Hit
GATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT800.11051555506437531No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT800.11051555506437531No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT800.11051555506437531No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT800.11051555506437531No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10913411062607062No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT790.10913411062607062No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT790.10913411062607062No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT790.10913411062607062No Hit
CCGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT790.10913411062607062No Hit
TCCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT780.10775266618776594No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT780.10775266618776594No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT780.10775266618776594No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT780.10775266618776594No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT780.10775266618776594No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT780.10775266618776594No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10637122174946124No Hit
GGCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT770.10637122174946124No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT770.10637122174946124No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT770.10637122174946124No Hit
CGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT770.10637122174946124No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT770.10637122174946124No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT770.10637122174946124No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT770.10637122174946124No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10637122174946124No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT760.10498977731115654No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT760.10498977731115654No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT760.10498977731115654No Hit
CGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT760.10498977731115654No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT760.10498977731115654No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10498977731115654No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10498977731115654No Hit
AGGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT760.10498977731115654No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10498977731115654No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10498977731115654No Hit
ATAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT760.10498977731115654No Hit
ACCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT760.10498977731115654No Hit
GCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT760.10498977731115654No Hit
CATCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT750.10360833287285184No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10360833287285184No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT750.10360833287285184No Hit
TATGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT750.10360833287285184No Hit
GCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT750.10360833287285184No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT750.10360833287285184No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT750.10360833287285184No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT740.10222688843454716No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10222688843454716No Hit
CTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT740.10222688843454716No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT730.10084544399624248No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT730.10084544399624248No Hit
CTTGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT730.10084544399624248No Hit
ATTGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT730.10084544399624248No Hit
GTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT730.10084544399624248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCCTA950.0295.000032
CAGCCCT157.212411E-6295.01
CGTTCCT157.212411E-6295.01
ATCCCCT400.0295.01
ACAGCCT550.0295.01
ATCCTCG108.44867E-4295.03
AGCCTAC3550.0295.03
TTGTCCT500.0295.01
TGGTCCT255.2568794E-10295.01
AATCCTA1850.0295.02
AACGCCT108.44867E-4295.01
TGCACCT303.6379788E-12295.01
CCAACCT550.0295.01
GCCTCCT157.212411E-6295.01
CCTACGA108.44867E-4295.05
CCTACCT206.145092E-8295.01
ATCTCCT550.0295.01
AGCTCCT206.145092E-8295.01
CACACCT303.6379788E-12295.01
TCGACCT500.0295.01
Produced by FastQC (version 0.11.5)