FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60320
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.14257294429708223No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.13594164456233424No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.13428381962864722No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.12102122015915119No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.12102122015915119No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.11604774535809018No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.11604774535809018No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.1143899204244032No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC680.11273209549071618No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC680.11273209549071618No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.11107427055702918No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.11107427055702918No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.11107427055702918No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.10941644562334218No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.10941644562334218No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC660.10941644562334218No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC640.10610079575596816No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC640.10610079575596816No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.10444297082228117No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.10444297082228117No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.10444297082228117No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC620.10278514588859416No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC620.10278514588859416No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC620.10278514588859416No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC610.10112732095490717No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC610.10112732095490717No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC610.10112732095490717No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC610.10112732095490717No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC610.10112732095490717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA950.0295.000032
TGATCCT206.1367246E-8295.01
TCGCCCT108.445184E-4295.01
ACAACCT108.445184E-4295.01
CGGCGGC108.445184E-4295.09
GTCGCCT108.445184E-4295.01
GTACCCT157.2064577E-6295.01
CGAACCT255.2568794E-10295.01
GCGACCT157.2064577E-6295.01
TAGACCT303.6379788E-12295.01
ACCGCCT450.0295.01
GGTCCTA900.0295.02
CGCGCCT206.1367246E-8295.01
GGATCCT206.1367246E-8295.01
GCGCCTA900.0295.02
TCCTCGG157.2064577E-6295.04
GCGCCCT255.2568794E-10295.01
CGTTCCT157.2064577E-6295.01
TGCGCCT206.1367246E-8295.01
CTACGGC108.445184E-4295.06
Produced by FastQC (version 0.11.5)