FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54706
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC550.10053741819910066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCCT350.0295.000031
CCGTCCT350.0295.000031
ACTACCT350.0295.000031
TCCACCT350.0295.000031
GGGCCTA700.0295.000032
ACGTCCT350.0295.000031
GGACCTA700.0295.000032
CACCCTA700.0295.000032
ATCGCCT350.0295.000031
GCCCCTA750.0295.02
GCCCCCT157.2028033E-6295.01
AGTCCTA900.0295.02
GATACCT303.6379788E-12295.01
AGTCCCT157.2028033E-6295.01
CAAACCT157.2028033E-6295.01
TGCCCCT303.6379788E-12295.01
AGATCCT157.2028033E-6295.01
GCGACCT255.2386895E-10295.01
TCCGCCT303.6379788E-12295.01
GTACAGG157.2028033E-6295.0295
Produced by FastQC (version 0.11.5)