FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108441
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT350.0295.01
TCTGCCT108.454474E-4295.01
AGTCCTA1850.0295.02
GATACCT500.0295.01
AGATCCT600.0295.01
CGAACCT600.0295.01
ATCTAGG255.275069E-10295.0295
ATCTACG108.454474E-4295.03
GTTTCCT206.15928E-8295.01
CAACGGG157.222323E-6295.06
CTTCCCT305.456968E-12295.01
CAGCCTA1500.0295.02
CGGCCTA1050.0295.02
AGCCCTA1500.0295.02
GAGTCCT450.0295.01
TAGACCT750.0295.01
GGCACCT600.0295.01
CCGGCCT255.275069E-10295.01
GATCCTA1450.0295.02
GGTCCTA1650.0295.02
Produced by FastQC (version 0.11.5)