FastQCFastQC Report
Wed 8 May 2024
EGAF00005491220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76699
Total Bases23 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6700.8735446355232793No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5610.731430657505313No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5560.7249116676879751No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5440.709266092126364No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5380.7014433043455586No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5290.6897091226743504No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5090.6636331634049988No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4770.6219116285740361No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4750.619304032647101No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4560.594531871341217No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4460.5814938917065412No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4350.5671521141083977No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4150.5410761548390461No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4000.5215191853870325No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3960.516303993533162No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3940.513696397606227No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3820.4980508220446159No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3790.49413942815421324No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3780.49283563019074567No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3690.4811014485195374No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3660.4771900546291346No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3660.4771900546291346No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3460.45111409535978303No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3320.4328609238712369No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3290.42894952998083413No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3290.42894952998083413No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3110.4054811666384177No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3070.40026597478454734No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3070.40026597478454734No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3040.39635458089414466No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2990.3898355910768067No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2940.3833166012594688No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2870.37419001551519576No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2840.370278621624793No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2830.36897482366132545No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2780.36245583384398755No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2780.36245583384398755No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2700.35202545013624686No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2660.34681025828237655No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2520.32855708679383044No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2470.3220380969764925No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2440.31812670308608976No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2420.3155191071591546No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2410.314215309195687No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2390.31160771326875186No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2390.31160771326875186No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2360.3076963193783491No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2330.3037849254879464No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2310.30117732956101123No Hit
GGACTACCGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2310.30117732956101123No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2300.29987353159754365No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2260.29465833974367334No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2250.29335454178020576No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2230.2907469458532706No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2160.28162036010899755No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2160.28162036010899755No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2130.27770896621859475No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2090.27249377436472444No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2070.2698861784377893No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2050.2672785825108541No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2020.2633671886204514No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2010.2620633906569838No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2010.2620633906569838No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1990.25945579473004865No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1960.2555444008396459No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1950.2542406028761783No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1950.2542406028761783No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1890.24641781509537283No Hit
GGACTACAGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1880.24511401713190523No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1820.23729122935109975No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1810.23598743138763217No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1800.23468363342416457No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1780.23207603749722944No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1780.23207603749722944No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1780.23207603749722944No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1760.22946844157029425No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1690.2203418558260212No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1680.21903805786255365No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1670.21773425989908604No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1650.21512666397215086No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1590.20730387619134538No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1560.20339248230094265No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1550.20208868433747507No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1550.20208868433747507No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1540.20078488637400746No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1540.20078488637400746No Hit
GGACTACAAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1540.20078488637400746No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1520.19817729044707233No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1510.19687349248360472No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1510.19687349248360472No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1500.19556969452013714No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1490.19426589655666957No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1450.18905070470279928No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1450.18905070470279928No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1450.18905070470279928No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1440.18774690673933167No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1440.18774690673933167No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1430.1864431087758641No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1420.1851393108123965No Hit
GGACTACCGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1400.18253171488546135No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1390.18122791692199378No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1380.17992411895852617No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1360.177316523031591No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1350.17601272506812343No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1340.17470892710465585No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1340.17470892710465585No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1330.17340512914118827No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1290.16818993728731796No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1280.16688613932385038No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1270.1655823413603828No Hit
GGACTACACGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1230.16036714950651246No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1230.16036714950651246No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1220.15906335154304488No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1220.15906335154304488No Hit
GGACTACCGGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1200.15645575561610972No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1190.15515195765264217No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1170.15254436172570698No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1170.15254436172570698No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1160.1512405637622394No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1160.1512405637622394No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1150.14993676579877183No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1140.14863296783530425No Hit
GGACTACCAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1120.14602537190836906No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1110.1447215739449015No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1110.1447215739449015No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1100.1434177759814339No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1100.1434177759814339No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1070.13950638209103117No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1070.13950638209103117No Hit
GGACTACAAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1070.13950638209103117No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.1382025841275636No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1050.136898786164096No Hit
GGACTACAGGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA1050.136898786164096No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1050.136898786164096No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1030.13429119023716085No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.12516460449288777No Hit
GGACTACTAGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA960.12516460449288777No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG950.1238608065294202No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA950.1238608065294202No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA940.12255700856595261No Hit
GGACTACTGGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA930.12125321060248505No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA910.11864561467554988No Hit
GGACTACTCGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA910.11864561467554988No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA910.11864561467554988No Hit
GGACTACCCGGGTATCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA910.11864561467554988No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC900.11734181671208228No Hit
GGACTACAAGGGTATCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG900.11734181671208228No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.11734181671208228No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA900.11734181671208228No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC900.11734181671208228No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA890.11603801874861472No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC880.11473422078514713No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.11343042282167956No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA860.11212662485821197No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC860.11212662485821197No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA850.11082282689474439No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.10951902893127682No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.10951902893127682No Hit
GGACTACACGGGTATCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG840.10951902893127682No Hit
GGACTACACGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA830.10821523096780923No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC800.1043038370774065No Hit
GGACTACCAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG800.1043038370774065No Hit
GGACTACCAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA790.1030000391139389No Hit
GGACTACTAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA790.1030000391139389No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC790.1030000391139389No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC790.1030000391139389No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA790.1030000391139389No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC780.10169624115047132No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.10169624115047132No Hit
GGACTACAAGGGTATCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA780.10169624115047132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)